Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars

Genomic DNA was extracted from tissue specimens of wild boars (Sus scrofa) in 10 different locations Mongolia. D-loop part genome amplified by PCR and subjected to sequencing. Determined sequences 18 were registered with the GenBank accession numbers obtained. A total 54 complete mitochondrial available NCBI taken as a reference for comparison that Mongolian boars. Sequence alignment, detection parsimonious informative sites, model selection, calculation nucleotide distances, Maximum Likelihood (ML) phylogenetic tree construction 1000 bootstrapped replications conducted using MEGA X software. trees constructed Hasegawa-Kishino-Yano (HKY) model. The results study showed geographic location played an important role sequence divergence between various locations. Most them grouped together according their respective locations, except several individuals. It is highly likely subpopulation boars, such S. scrofa raddianus nigripes, have had same ancestor. In order fully evaluate distribution, ecology, biology it essential compare supplemental gene can reveal differences populations neighboring areas, Russia, northeast China, Kazakhstan. this will be useful protecting conserving